内质网
高尔基体
细胞生物学
分泌途径
生物
鸟嘌呤核苷酸交换因子
GTP酶
亚细胞定位
分泌蛋白
拟南芥
膜泡运输蛋白质类
胞吐
细胞器
蛋白质亚细胞定位预测
液泡
小泡
化学
分泌物
内体
转运蛋白
拉布
内膜系统
细胞质
内吞作用
膜蛋白
突触融合蛋白
细胞内
生物化学
基因
突变体
液泡蛋白分选
作者
Carine De Marcos Lousa,Eric Soubeyrand,Paolo Bolognese,Valérie Wattelet-Boyer,Guillaume Bouyssou,Claire-Line Marais,Yohann Boutté,F. Filippini,Patrick Moreau
摘要
SNARE proteins are central elements of the machinery involved in membrane fusion of eukaryotic cells. In animals and plants, SNAREs have diversified to sustain a variety of specific functions. In animals, R-SNARE proteins called brevins have diversified; in contrast, in plants, the R-SNARE proteins named longins have diversified. Recently, a new subfamily of four longins named 'phytolongins' (Phyl) was discovered. One intriguing aspect of Phyl proteins is the lack of the typical SNARE motif, which is replaced by another domain termed the 'Phyl domain'. Phytolongins have a rather ubiquitous tissue expression in Arabidopsis but still await intracellular characterization. In this study, we found that the four phytolongins are distributed along the secretory pathway. While Phyl2.1 and Phyl2.2 are strictly located at the endoplasmic reticulum network, Phyl1.2 associates with the Golgi bodies, and Phyl1.1 locates mainly at the plasma membrane and partially in the Golgi bodies and post-Golgi compartments. Our results show that export of Phyl1.1 from the endoplasmic reticulum depends on the GTPase Sar1, the Sar1 guanine nucleotide exchange factor Sec12, and the SNAREs Sec22 and Memb11. In addition, we have identified the Y48F49 motif as being critical for the exit of Phyl1.1 from the endoplasmic reticulum. Our results provide the first characterization of the subcellular localization of the phytolongins, and we discuss their potential role in regulating the secretory pathway.
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