抗菌剂
壳聚糖
Zeta电位
微生物学
生物膜
多重耐药
大肠杆菌
铜绿假单胞菌
甘露糖
金黄色葡萄球菌
材料科学
抗生素
细菌
化学
生物
纳米技术
纳米颗粒
生物化学
基因
遗传学
作者
Sadaf Ejaz,Ayesha Ihsan,Tayyaba Nооr,Saima Shabbir,Muhammad Imran
出处
期刊:Polymer Testing
[Elsevier]
日期:2020-11-01
卷期号:91: 106814-106814
被引量:27
标识
DOI:10.1016/j.polymertesting.2020.106814
摘要
Escalating antimicrobial resistance is causing a major threat to the public health. Failure of traditional antibiotics urges the development of alternative therapeutics, which include biopolymeric nanosystems with intrinsic antimicrobial potential. In the present study, mannose functionalized chitosan nanosystems (M-CNS) were fabricated through reductive amination of chitosan with mannose and further its ionic gelation. Changes in zeta potential and characteristic peaks in FTIR spectra revealed surface functionalization of chitosan with mannose. Zeta-sizer studies disclosed relatively higher size (180 ± 5 nm) of mannosylated CNS as compared to CNS (162 ± 7 nm). Inversely, the zeta-potential was reduced from +32.2 mV to +25.4 mV for M-CNS. Scanning electron microscopy verified the slight increase in size for M-CNS. Antimicrobial evaluation of designed nanosystems as alternative antibacterial agent was assessed by time-kill, polystyrene adherence and antibiofilm assays against both Gram-positive and Gram-negative pathogens. Results indicated that mannose functionalized CNS inhibited the growth of resistant Escherichia coli and Listeria monocytogenes, while demonstrating anti-adherence and biofilm disruption activity. Furthermore, highly resistant Pseudomonas aeruginosa and Staphylococcus aureus were also susceptible against M-CNS. This study unveiled the potential of M-CNS against pathogenic, multidrug resistant, biofilm forming bacteria; thus, making them an ideal candidate for developing alternative-medicines to cure the emerging resistant infections.
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