化学
肝细胞癌
微流控芯片
微流控
抗体
循环肿瘤细胞
癌
纳米技术
内科学
癌症研究
癌症
医学
免疫学
生物
材料科学
转移
作者
Lin Zhu,Huibin Lin,Shuang Wan,Xiaofeng Chen,Lingling Wu,Zhi Zhu,Yanling Song,Bin Hu,Chaoyong Yang
出处
期刊:Analytical Chemistry
[American Chemical Society]
日期:2020-10-30
卷期号:92 (22): 15229-15235
被引量:22
标识
DOI:10.1021/acs.analchem.0c03936
摘要
As a malignant disease that seriously threatens human health, hepatocellular carcinoma (HCC) lacks effective early screening and prognostic assessment methods. Herein, we developed a method for efficient capture and multiphenotype analysis of circulating tumor cells (CTCs) of hepatocellular carcinoma. The anti-ASGPR antibody and the anti-EpCAM antibody were modified in parallel on a deterministic lateral displacement (DLD)-patterned microfluidic Synergetic-Chip to enhance capture efficiency by a complementary effect. CTCs were detected in 45 out of 45 (100%) HCC patients, with a sensitivity and specificity of 97.8 and 100%, respectively. Patients with more total CTCs and nonepithelial CTCs were in later stages of HCC and had more malignant progression. This strategy proposes a feasible approach for early diagnosis and prognosis of hepatocellular carcinoma.
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