Peroxotungstate oxidation-mediated two-phase amplification system (POM-TPAS) for bisulfite-free quantification of locus-specific 5-hydroxymethylcytosine

The peroxotungstate oxidation-mediated two-phase amplification system (POM-TPAS) is applied for sensitive detection of locus-specific 5hmC in DNA with low non-specific amplification.