Simultaneous determination of eight furocoumarines in cosmetics byhighperformance liquid chromatography and verification byliquid chromatography tandem mass spectrometry

A method using high performance liquid chromatography(HPLC)and liquid chromatography-tandem mass spectrometry(LC-MS/MS)was developed for the simultaneous determination of eight furocoumarines(8-hydroxypsoralen,psoralen,isopsoralen,8-methoxypsoralen,5-methoxypsoralen,trioxsalen,imperatorin and isoimperatorin)in cosmetics.The cosmetic samples,including cream,lotion,shampoo,powder and lipstick,were supersonically extracted with appropriate solvents.The extract was centrifuged,and the supernatant was filtered through a membrane,and then separated on an Agilent Zorbax SB-Phenyl chromatographic column(250 mm×4.6 mm,5 μm)by gradient elution at a flow rate of 1.0 mL/min with methanol-acetonitrile-water as mobile phases.The column temperature was set at 30 ℃.The wavelength of detection was 250 nm.The limits of quantification(LOQs)were 0.25 mg/kg for 8-hydroxypsoralen and 0.5 mg/kg for psoralen,isopsoralen,8-methoxypsoralen,5-methoxypsoralen,trioxsalen,imperatorin and isoimperatorin.The recoveries at three spiked levels were in the range of 85.0%-105.8% with the relative standard deviations(RSDs)of 0.41%-7.90%.The intra-day precision(n=6)was less than 1%,and the inter-day precision(n=6)was less than 2%for the peak areas of the eight furocoumarines in a mixed standard solution.The method is accurate,simple,rapid and suitable for the determination of the eight furocoumarines in various cosmetic samples.