血管紧张素II
小RNA
生物
折叠变化
微阵列
腹主动脉瘤
微阵列分析技术
细胞凋亡
基因表达谱
实时聚合酶链反应
信号转导
分子生物学
基因表达
细胞生物学
癌症研究
基因
受体
医学
遗传学
动脉瘤
外科
作者
Jiaoni Wang,Huankun Sun,Yingying Zhou,Kaiyu Huang,Jia-Qun Que,Yu Peng,Jinsheng Wang,Cong Lin,Yan Xue,Kangting Ji
摘要
Abstract Abdominal aortic aneurysm (AAA) is an unpredictable but lethal disease that poses a therapeutic dilemma. Circular RNAs (circRNAs), whose functional roles as transcriptional regulators and microRNA (miRNA) sponges have been shown in former studies, are potential biomarkers for many diseases. AAA in male C57BL/6 J mice was induced by coadministration of angiotensin II (Ang II) and 3,4‐benzopyrene (BaP). The circRNA expression profiling was performed using two samples from the control group and two samples from the AAA group. Quantitative real‐time polymerase chain reaction (qRT‐PCR) was used to confirm the reliability of the microarray results. Among the 14 236 detected circRNAs, 413 showed obvious expression changes (fold change ≥ 2; P < 0.05) between the BaP/Ang II‐induced AAA group and control group. Of the 413 that showed significant changes, 271 were upregulated, while the other 142 were downregulated. The expression levels of 10 circRNAs were validated by qRT‐PCR. The interactions of the differentially expressed circRNAs with miRNAs were predicted. Immunofluorescence showed prominent vascular smooth muscle cell apoptosis in abdominal aortic tissues in the BaP/Ang II group. Furthermore, a circRNA‐miRNA coexpression network based on six apoptosis‐related circRNAs was built. The genes regulated by the network mapped to several pathways, including apoptosis, the IL‐17 signaling pathway, and vascular endothelial growth factor signaling pathway, all of which are related to AAA formation. This study performed circRNA expression profiling in AAA and the results specifically predicted the regulatory role of circRNAs in AAA pathogenesis.
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