生物分子
纳米技术
生物物理学
化学
荧光
荧光显微镜
合成生物学
计算生物学
计算机科学
生物
材料科学
物理
量子力学
作者
Graham C. R. Ellis‐Davies
出处
期刊:Nature Methods
[Springer Nature]
日期:2007-07-30
卷期号:4 (8): 619-628
被引量:829
摘要
Caged compounds are light-sensitive probes that functionally encapsulate biomolecules in an inactive form. Irradiation liberates the trapped molecule, permitting targeted perturbation of a biological process. Uncaging technology and fluorescence microscopy are 'optically orthogonal': the former allows control, and the latter, observation of cellular function. Used in conjunction with other technologies (for example, patch clamp and/or genetics), the light beam becomes a uniquely powerful tool to stimulate a selected biological target in space or time. Here I describe important examples of widely used caged compounds, their design features and synthesis, as well as practical details of how to use them with living cells.
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