生物
计算生物学
基因
遗传学
发起人
抄写(语言学)
抑制因子
翻译(生物学)
转录因子
基因表达
核糖体分析
核糖核酸
细胞生物学
增强子
转录组
真核翻译
信使核糖核酸
语言学
哲学
作者
Vivek K. Mutalik,Joao C. Guimaraes,Guillaume Cambray,Colin Lam,Marc Juul Christoffersen,Quynh-Anh Mai,Andrew H. Tran,Morgan L Paull,Jay D. Keasling,Adam P. Arkin,Drew Endy
出处
期刊:Nature Methods
[Springer Nature]
日期:2013-04-01
卷期号:10 (4): 354-360
被引量:604
摘要
An inability to reliably predict quantitative behaviors for novel combinations of genetic elements limits the rational engineering of biological systems. We developed an expression cassette architecture for genetic elements controlling transcription and translation initiation in Escherichia coli: transcription elements encode a common mRNA start, and translation elements use an overlapping genetic motif found in many natural systems. We engineered libraries of constitutive and repressor-regulated promoters along with translation initiation elements following these definitions. We measured activity distributions for each library and selected elements that collectively resulted in expression across a 1,000-fold observed dynamic range. We studied all combinations of curated elements, demonstrating that arbitrary genes are reliably expressed to within twofold relative target expression windows with ∼93% reliability. We expect the genetic element definitions validated here can be collectively expanded to create collections of public-domain standard biological parts that support reliable forward engineering of gene expression at genome scales.
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