Enzyme-linked immunosorbent assay (ELISA): the basics

医学 梅德林 生物化学 生物
作者
Karishma Shah,Panagiotis Maghsoudlou
出处
期刊:British journal of hospital medicine [Mark Allen Group]
卷期号:77 (7): C98-C101 被引量:108
标识
DOI:10.12968/hmed.2016.77.7.c98
摘要

British Journal of Hospital MedicineVol. 77, No. 7 Core Training for DoctorsEnzyme-linked immunosorbent assay (ELISA): the basicsKarishma Shah, Panagiotis MaghsoudlouKarishma ShahSearch for more papers by this author, Panagiotis MaghsoudlouSearch for more papers by this authorKarishma Shah; Panagiotis MaghsoudlouPublished Online:7 Jul 2016https://doi.org/10.12968/hmed.2016.77.7.C98AboutSectionsView articleView Full TextPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareShare onFacebookTwitterLinked InEmail View article References Avrameas S (1969) Coupling of enzymes to proteins with glutaraldehyde. Use of the conjugates for the detection of antigens and antibodies. Immunochemistry 6: 43–52 Crossref, Medline, Google Scholarde la Rica R, Stevens MM (2012) Plasmonic ELISA for the ultrasensitive detection of disease biomarkers with the naked eye. Nat Nanotechnol 7: 821–4 (doi: https://doi.org/10.1038/nnano.2012.186) Crossref, Medline, Google ScholarEngvall E, Perlmann P (1971) Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry 8: 871–4 Crossref, Medline, Google ScholarHock BD, Stamp LK, Hayman MW, Keating PE, Helms ET, Barclay ML (2015) Development of an ELISA based competitive binding assay for the analysis of drug concentration and anti-drug antibody levels in patients receiving adalimumab or infliximab. Ther Drug Monit 38: 32-41 (doi: https://doi.org/10.1097/FTD.0000000000000229) Crossref, Google ScholarKaushik A, Tiwari S, Dev Jayant R, Marty A, Nair M (2016) Towards detection and diagnosis of Ebola virus disease at point-of-care. Biosens Bioelectron 75: 254–72 (doi: https://doi.org/10.1016/j.bios.2015.08.040) Crossref, Medline, Google ScholarMurdock RC, Shen L, Griffin DK, Kelley-Loughnane N, Papautsky I, Hagen JA (2013) Optimization of a paper-based ELISA for a human performance biomarker. Anal Chem 85: 11634–42 (doi: https://doi.org/10.1021/ac403040a) Crossref, Medline, Google ScholarVan Weemen BK, Schuurs AH (1971) Immunoassay using antigen-enzyme conjugates. FEBS Lett 15: 232–6 Crossref, Medline, Google ScholarYalow RS, Berson SA (1960) Immunoassay of endogenous plasma insulin in man. J Clin Invest 39: 1157–75 (doi: https://doi.org/10.1172/JCI104130) Crossref, Medline, Google Scholar FiguresReferencesRelatedDetailsCited byViral T-cell epitopes – Identification, characterization and clinical applicationSeminars in Immunology, Vol. 66Droplet Surface Immunoassay by Relocation (D-SIRe) for High-Throughput Analysis of Cytosolic Proteins at the Single-Cell Level23 February 2023 | Analytical ChemistryMagnetically localized and wash-free fluorescence immunoassay (MLFIA): proof of concept and clinical applications1 January 2023 | Lab on a Chip, Vol. 23, No. 4Supramolecular Phenylalanine-Derived Hydrogels for the Sustained Release of Functional Proteins24 January 2023 | ACS Biomaterials Science & Engineering, Vol. 9, No. 2Determination of Nicotinic Acid in Food and Pharmaceuticals by a Simple and Rapid Fluorescence Polarization Immunoassay (FPIA)31 January 2023 | Analytical LettersVitamin D metabolites and analytical challenges1 January 2023 | Analytical Methods, Vol. 15, No. 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