精氨酸酶
巨噬细胞
甘露糖受体
M2巨噬细胞
肾
细胞生物学
炎症
促炎细胞因子
内分泌学
内科学
生物
化学
免疫学
医学
体外
生物化学
精氨酸
氨基酸
作者
Nara Shin,Arnaud Marlier,Leyuan Xu,Natnael Doilicho,Daniel Linberg,Jian‐Kan Guo,Lloyd G. Cantley
出处
期刊:Journal of The American Society of Nephrology
日期:2022-06-01
卷期号:33 (6): 1077-1086
被引量:10
标识
DOI:10.1681/asn.2021121548
摘要
After kidney injury, macrophages transition from initial proinflammatory activation to a proreparative phenotype characterized by expression of arginase-1 (Arg1), mannose receptor 1 (Mrc1), and macrophage scavenger receptor 1 (Msr1). The mechanism by which these alternatively activated macrophages promote repair is unknown.We characterized the macrophage and renal responses after ischemia-reperfusion injury with contralateral nephrectomy in LysM-Cre;Arg1fl/fl mice and littermate controls and used in vitro coculture of macrophages and tubular cells to determine how macrophage-expressed arginase-1 promotes kidney repair.After ischemia-reperfusion injury with contralateral nephrectomy, Arg1-expressing macrophages were almost exclusively located in the outer stripe of the medulla adjacent to injured S3 tubule segments containing luminal debris or casts. Macrophage Arg1 expression was reduced by more than 90% in injured LysM-Cre;Arg1fl/fl mice, resulting in decreased mouse survival, decreased renal tubular cell proliferation and decreased renal repair compared with littermate controls. In vitro studies demonstrate that tubular cells exposed apically to dead cell debris secrete high levels of GM-CSF and induce reparative macrophage activation, with those macrophages in turn secreting Arg1-dependent factor(s) that directly stimulate tubular cell proliferation.GM-CSF-induced, proreparative macrophages express arginase-1, which is required for the S3 tubular cell proliferative response that promotes renal repair after ischemia-reperfusion injury.
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