Protective effects of the phenolic compounds from mung bean hull against H2O2-induced skin aging through alleviating oxidative injury and autophagy in HaCaT cells and HSF cells

To add value to food waste and seek skin aging suppressor, petroleum ether, ethyl acetate, n-butanol and water phenolic extracts were produced from mung bean hulls subjected to ultrasound-assisted ethanolic extraction. The four extracts all contained protocatechuic acid, isovitexin, vitexin, caffeic acid, 4-coumaric acid, ferulic acid, rutin and chlorogenic acid (revealed by UHPLC-MS/MS). The effects of the four extracts and their main phenolic compounds against H2O2-caused cell damage and aging in HaCaT and HSF cells were examined (including cell viability, ROS, MDA, SOD, GSH-px and β-galactosidase levels). The four extracts and the eight phenolic compounds exhibited different protective effects on H2O2-treated HaCaT/HSF cells viability, with the ethyl acetate extract among the extracts, and isovitexin and vitexin among the eight compounds, exerting the greatest protection. Therefore, isovitexin and vitexin may be the key oxidative stress and autophagy modulators of mung bean hull, and they inhibit skin aging and damage likely through suppressing Nrf2/keap1/HO-1 related oxidative damage and LC3II/p62/GATA4 related autophagy.