Biodegradation of imidacloprid: Molecular and kinetic analysis

Imidacloprid (C9H10ClN5O2) is the most widely used insecticide. Its persistence and toxic nature have caused a detrimental effect on living biota. Thus its removal from the contaminated environment has become imperative. The present study aimed to isolate bacterial species from pesticide-contaminated sites and assess their potential for biodegradation of imidacloprid. The 16S rRNA analysis revealed the genetic relatedness of isolates to Sphingobacterium sp., Agrobacterium sp., Pseudomonas sp., and Bacillus sp. Batch biodegradation studies showed that Sphingobacterium sp. and Agrobacterium sp. were the most promising isolates as they degraded 81.0% and 84.9%, respectively, of imidacloprid at the concentration of 95 mg/L via co-metabolism. Kinetic study (Vmax/Ks ratio) also suggested the high degradation efficiency of these isolates. Imidacloprid-guanidine (C9H11ClN4) was identified as the metabolite. This report highlights the potential of bacteria for imidacloprid degradation and could be utilized for the formulation of strategies for the remediation of imidacloprid contaminated environments.