化学
多角度光散射
抗氧化剂
多糖
共价键
色谱法
回转半径
有机化学
光散射
光学
物理
散射
聚合物
作者
Xue Chen,Yiling Tian,Zhang Jie,LI Yue-qiu,Wenhui Zhang,Jing Zhang,Yuwei Dou,Haiyang Dou
出处
期刊:Food Chemistry
[Elsevier]
日期:2022-08-01
卷期号:384: 132619-132619
被引量:5
标识
DOI:10.1016/j.foodchem.2022.132619
摘要
Oxidation is an essential biological process for human life. In this study, low density lipoprotein-Tremella fuciformis polysaccharide (LDL-TFP) complexes were prepared by electrostatic and covalent methods. The effects of preparation method on the structure and antioxidant activity of LDL-TFP complexes were investigated by asymmetrical flow field-flow fractionation (AF4) coupled with ultraviolet-visible (UV/Vis), multiangle light scattering (MALS), and differential refractive index (dRI) detectors. The results showed that the electrostatic LDL-TFP complexes had a spherical structure, while the covalent LDL-TFP complexes had a rod-like structure as indicated by the ratio of Rg (radius of gyration) to Rh (hydrodynamic radius). Moreover, the results revealed that the antioxidant activity of the LDL-TFP complexes on the HepG2 could be related to the structure of LDL-TFP complexes. The antioxidant activity of LDL-TFP complexes formed by LDL modified with phospholipase A2 was further enhanced. This study would help expand the application of TFP.
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