Enhancement of diazinon degradation and chemical analysis of metabolites

In this study, diazinon high degradation was achieved by using Ethyl methane sulfonate (EMS) as a chemical mutagen, 38.19% of diazinon was degradedin the minimal media as compared to 1.83% in control media after 5 days.Due to the first step of ethylmethane sulphonate (EMS) chemical treatment, diazinon degradation increased to 62.19% which was subsequently increased to 86.21% after the EMS treatment second step. Analysis of organophosphorus hydrolaseprotein involved in diazinon hydrolysis revealed that itis a member of the MBL-fold metallo hydrolase superfamily and has beta lactamase fold.2-Iso-4-methyl-6- hydroxypyrimidine (IMHP) was detected as the main degradation product of diazinon through GC/MS analysis after 5days of incubation and further metabolized to unknown polar metabolites that weredetected using GC/MS after 10 days.