引导RNA
计算生物学
基因组工程
生物
DNA
转录激活物样效应核酸酶
基因靶向
作者
Hiroshi Nishimasu,Xi Shi,Soh Ishiguro,Linyi Gao,Seiichi Hirano,Sanae Okazaki,Taichi Noda,Omar O. Abudayyeh,Jonathan S. Gootenberg,Hideto Mori,Seiya Oura,Benjamin Holmes,Mamoru Tanaka,Motoaki Seki,Hisato Hirano,Hiroyuki Aburatani,Ryuichiro Ishitani,Masahito Ikawa,Nozomu Yachie,Feng Zhang,Osamu Nureki
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:2018-09-21
卷期号:361 (6408): 1259-1262
被引量:653
标识
DOI:10.1126/science.aas9129
摘要
Expanding the targeting space of Cas9 CRISPR-Cas9 associates with a guide RNA to target and cleave a specific DNA site next to a protospacer adjacent motif (PAM). Streptococcus pyogenes Cas9 (SpCas9), the one most often used for genome editing, only recognizes the NGG sequence (where N is any nucleobase) as the PAM, which restricts regions in the genome that can be targeted. To address this limitation, Nishimasu et al. created a SpCas9 variant that recognizes NG rather than NGG. The SpCas9-NG variant increased the targeting range, had a specificity similar to that of the wild-type enzyme, and could be used with a base editor. Thus, SpCas9-NG is a powerful addition to the CRISPR-Cas9 genome engineering toolbox and will be useful in a broad range of applications, from basic research to clinical therapeutics. Science , this issue p. 1259
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