上转换纳米颗粒侧向层析检测技术研究进展

Abstract： Upconversion nanoparticle lateral flow assay (UCNPs-LFA) is a novel point-of-care testing (POCT) method that combines the unique optical properties of upconversion luminescent nanomaterials with the convenience of an immunochromatographic platform， offering advantages such as high sensitivity and low background interference.This article provides a systematic review of the technical principles of UCNPs-LFA， strategies for material synthesis and surface functionalization， methods for optimizing detection performance， and the latest research advances in fields such as biomedical diagnostics， food safety analysis， and environmental monitoring.Through the anti-Stokes emission mechanism induced by near-infrared light excitation， UCNPs effectively overcome the limitations of traditional colloidal gold test strips， such as low sensitivity and susceptibility to matrix interference.This paper further explores the role of optimization strategies-such as core-shell structure regulation， surface modification， and signal amplification-in enhancing detection performance， and summarizes application cases of this technology in the detection of various targets.Finally， it outlines the future development directions of UCNPs-LFA toward intelligent， portable， and multi-target simultaneous detection， highlighting its key potential in driving the evolution of POCT technology toward precision， quantification， and networking.Keywords： upconversion nanoparticles； lateral flow assay； point-of-care testing； luminescent labels； biosensing 文章编号： 1000