诱导多能干细胞
SOX2
重编程
同源盒蛋白纳米
KLF4公司
胚胎干细胞
林28
生物
移植
干细胞
细胞分化
癌症研究
病理
医学
免疫学
细胞生物学
细胞
内科学
遗传学
基因
作者
Yixuan Zhang,Liping Liu,Mi Li,Jianling Huang,Hui Xu,Xiaodong Chen,Weiwei Zhu,Yunpeng Cai,Ningning Guo,Zhiqiang Chen,Yun‐Wen Zheng,Yumei Li
标识
DOI:10.1016/j.transproceed.2018.04.008
摘要
Presently, interesting research related to induced pluripotent stem cells (iPSCs) is emerging. However, the development of new therapies and techniques for treatment of refractory diseases is still required in dermatology. We are exploring novel methods to provide stem cell therapy and elucidate research mechanisms underlying troublesome diseases by reprogramming iPSCs from the fibroblasts of keloid lesions from patients in vitro.Here, we identified the expression of fibroblastic genes in the fibroblast derived from diseased individuals. Corresponding iPSCs were then produced by transfecting patient fibroblasts with non-modified RNA cocktails, expressing OCT4, SOX2, KLF4, cMYC, NANOG, and LIN28 reprogramming factors. The pluripotency of these patient-derived iPSCs was identified by immunocytochemistry, real-time quantitative polymerase chain reaction, and teratoma formation in vivo in non-obese diabetic/severe combined immunodeficiency mice.All iPSCs derived from patients significantly expressed the pluripotent transcription factors and could be expanded in vitro. Furthermore, induction of terminal differentiation in long-term culture and the capability of forming embryonic bodies to differentiate into all 3 germ layers in vivo were confirmed in immune-deficient mice.Fibroblasts from a keloid patient were successfully reprogrammed to iPSCs in vitro. This reprogramming may provide a basis for the production of individualized modified artificial skin to prevent rejections after xenogeneic skin transplantation and trauma through autologous skin transplantation. These cells can also offer a new platform for research on mechanisms underlying skin diseases and personal medical applications.
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