生物
微泡
小RNA
外体
细胞生物学
血淋巴
基因
岩松珠母贝
基因表达
遗传学
生物化学
珍珠
珍珠牡蛎
哲学
神学
作者
Zhe Zheng,Zhijie Xu,Caixia Cai,Yongshan Liao,Chuangye Yang,Xiaodong Du,Ronglian Huang,Yuewen Deng
标识
DOI:10.1016/j.cbd.2022.101004
摘要
MicroRNAs (miRNAs) can efficiently regulate gene expression at intracellular and extracellular levels. Plant-derived miRNAs are highly enriched in animal haemolymph and regulate mammalian gene expression. However, evidence for food-derived miRNAs in Mollusca species is lacking. In this study, we fed the microalga Nannochloropsis oculata to the pearl oyster Pinctada fucata martensii and detected dietary miRNAs in exosomes isolated from the haemolymph by RNA-seq. In total, 273 endogenous miRNAs were identified in all biological replicates. We identified 23 microalgae-derived miRNAs in the exosomes of pearl oyster haemolymph. Most microalgae-derived miRNAs showed high expression levels in both exosomes and microalgae and exhibited apparent variation among individuals. These food-derived miRNAs were predicted to participate in endocytosis, apoptosis, signal transduction, energy metabolism, and biomineralization by targeting multiple genes. These findings demonstrated the cross-kingdom transport of miRNAs from microalgae to bivalves and provide insights into novel nutrient transmission through the food chain.
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