精氨酸酶
宫颈炎
宫颈上皮内瘤变
病毒载量
医学
宫颈癌
鳞状上皮内病变
内科学
癌症
免疫学
病理
癌症研究
病毒
生物
妇科
精氨酸
氨基酸
生物化学
作者
Moufida Souid,Randa Ghedira,Sameh Souissi,Nadia Bouzgarrou,Sallouha Gabbouj,Salwa Shini-Hadhri,Mohamed-Saleh Rhim,A. Boukadida,Dhekra Toumi,Raja Faleh,Noureddine Bouaouina,Abdelfattah Zakhama,Elham Hassen
出处
期刊:Immunobiology
[Elsevier]
日期:2022-03-01
卷期号:227 (2): 152189-152189
被引量:4
标识
DOI:10.1016/j.imbio.2022.152189
摘要
Little is known about the relationship between arginase, an immunosuppressive enzyme, and cervical lesions. The present study is aimed at evaluating arginase activity in plasma and mRNA arginase isoforms expression in cervical cells of patients with abnormal cytology and identifying their relationship with Human papillomavirus (HPV) related parameters such as: HPV type, HPV circulating viral load and anti-HPV16 IgG.This study included 77 women with cervical lesions and 95 matched controls. Arginase activity was detected by colorimetric assay. Arginase mRNA expression and HPV viral load were evaluated by quantitative real time PCR and anti-HPV16 antibodies were assessed by ELISA.Compared to controls, the arginase activity was higher among women with cervicitis / low grade squamous intraepithelial lesions (LSIL) (OR: 1.872, 95% CI: 0.833-4.210), and also among women with high-grade squamous intraepithelial lesions (HSIL) / squamous cell carcinoma (SCC) (OR: 3.358, 95% CI: 1.670-8.910). Compared to controls, mRNA expression was significantly upregulated in women with cervical cervicitis and SIL for ARG1, and in women with cancer lesions for ARG2. Arginase activity was positively correlated to ARG2 mRNA expression but not to ARG1. High arginase activity was associated with HPV16, high levels of HPV viral load, and low levels of anti-HPV16 antibodies.Our findings demonstrated that arginase activity and isoforms expression were enhanced in women with HPV-related precancerous lesions and cervical cancer. Further studies are needed to identify how arginase enzyme induces disease progression and severity.
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