超声
活力测定
抗菌剂
抗氧化剂
DPPH
聚乙二醇
化学
食品科学
核化学
材料科学
生物化学
色谱法
体外
有机化学
作者
Meenakshi Dudi,Raj Kumar,Priyanshi Saini,Añil Sharma,Satish Kumar Pandey,Harpreet Kaur,Rajneesh Kumar,S.K. Mehta
标识
DOI:10.1016/j.jddst.2023.104484
摘要
Osthole (OST) is a naturally occurring bioactive molecule mainly found in the medicinal plants; Cnidium monnieri and Angelica pubescens, which is known to has its exceptional therapeutic properties due to its unique structure and strong antioxidant potential. Herein, we prepared OST loaded oil (Corn/Lemon oil (1:1)) in water nanoemulsion (OST-NEm) using low cost and highly effective ultrasonication emulsification method. The obtained NEm was clear, spherical droplet-shaped with an average size of 65.32 nm. NEm displayed good storage stability for 60 days; Moreover NEm stability under variable salt (NaCl) concentration (100–500 mM) and different pH (2–7.4) conditions was also optimized. The toxicity of the NEm was reduced by using a biocompatible stabilizer d-α-Tocopherol polyethylene glycol 1000 succinate (TPGS). NEm exhibits 79.89% OST encapsulation efficiency and 64% cumulative release at pH 7.4. The cell viability studies of both pure and OST-NEm were tested on Caco-2 cells and confirmed no toxicity up to a concentration of 160 μM. The antioxidant potential of both pure OST and OST-NEm was measured with DPPH radical and the results were compared with the control butylated hydroxy toluene (BHT). The IC50 values for the OST, OST-NEm, pure NEm and BHT were found to be 17 ± 0.7, 22 ± 2.0, 27 ± 0, 15 ± 1.0 μM, respectively. Efforts have also been made to study the interactions between OST and riboflavin via photoluminescence spectroscopy to understand the location of the OST in NEm.
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