鼻息肉
医学
成纤维细胞
炎症
转录组
促炎细胞因子
免疫学
信号转导
肌成纤维细胞
细胞外基质
病理
纤维化
基因表达
癌症研究
细胞生物学
生物
基因
细胞培养
遗传学
作者
Cristina Porras‐González,José María Palacios-García,Serafín Sánchez‐Gómez,Juan Maza‐Solano,Gonzalo Álba,Víctor Sánchez‐Margalet,O Palorames,A del Cuvillo,J A Cordero-Varela,Ramón Moreno‐Luna,J L Muñoz-Bravo
出处
期刊:Rhinology
[Rhinology]
日期:2023-01-01
被引量:2
摘要
Background: Fibroblasts and others mesenchymal cells have recently been identified as critical cells triggering tissue-specific inflammatory responses. Persistent activation of fibroblasts inflammatory program has been suggested as an underlying cause of chronic inflammation in a wide range of tissues and pathologies. Nevertheless, the role of fibroblasts in the emergence of chronic inflammation in the upper airway has not been previously addressed. We aimed to elucidate whether fibroblasts could have a role in the inflammatory response in chronic rhinosinusitis with nasal polyps (CRSwNP). Methodology: We performed whole-transcriptome microarray in fibroblast cultured from CRSwNP samples and confirmed our results by qRT-PCR. We selected patients without other associated diseases in upper airway. To investigate shifts in transcriptional profile we used fibroblasts from nasal polyps and uncinate mucosae from patient with CRSwNP, and fibroblasts from uncinate mucosae from healthy subjects as controls. Results: This study exposes activation of a pro-inflammatory and pro-fibrotic transcriptional program in nasal polyps and CRSwNP fibroblasts when compared to controls. Our Gene-set Enrichment Analysis (GSEA) pointed to common up-regulation of several pro-inflammatory pathways in patients-derived fibroblasts, along with higher mRNA expression levels of cytokines, growth factors and extracellular matrix components. Conclusions: Our work reveals a potential new source of inflammatory signaling in CRSwNP. Furthermore, our results suggest that deregulated inflammatory signaling in tissue-resident fibroblasts could support a Type-2 inflammatory response. Further investigations will be necessary to demonstrate the functionality of these novel results.
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