铜蓝蛋白
背景(考古学)
流式细胞术
基因亚型
免疫系统
免疫荧光
平衡
淋巴细胞
生物
化学
免疫学
细胞生物学
分子生物学
生物化学
抗体
基因
古生物学
作者
João Banha,Liliana Marques,Rita Palmeira‐de‐Oliveira,Maria de Fátima Martins,Eleonora Paixão,Dina Pereira,Rui Malhó,Deborah Penque,Luciana Costa
标识
DOI:10.1016/j.freeradbiomed.2007.10.032
摘要
Ceruloplasmin (CP) is a multicopper oxidase involved in the acute phase reaction to stress. Although the physiological role of CP is uncertain, its role in iron (Fe) homeostasis and protection against free radical-initiated cell injury has been widely documented. Previous studies showed the existence of two molecular isoforms of CP: secreted CP (sCP) and a membrane glycosylphosphatidylinositol (GPI)-anchored form of CP (GPI-CP). sCP is produced mainly by the liver and is abundant in human serum whereas GPI-CP is expressed in mammalian astrocytes, rat leptomeningeal cells, and Sertolli cells. Herein, we show using RT-PCR that human peripheral blood lymphocytes (huPBL) constitutively express the transcripts for both CP molecular isoforms previously reported. Also, expression of CP in huPBL is demonstrated by immunofluorescence with confocal microscopy and flow cytometry analysis using cells isolated from healthy blood donors with normal Fe status. Importantly, the results obtained show that natural killer cells have a significantly higher CP expression compared to all other major lymphocyte subsets. In this context, the involvement of lymphocyte-derived CP on host defense processes via its anti/prooxidant properties is proposed, giving further support for a close functional interaction between the immune system and the Fe metabolism.
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