骨骼肌
胰高血糖素
环化酶
内科学
腺苷酸激酶
内分泌学
化学
胰岛素
肽
受体
膜
生物化学
生物
医学
作者
Elena Delgado,Miguel Ángel Limón Luque,Ana Alcántara,M. A. Trapote,Felipe Clemente,C. Galera,Isabel Valverde,María Luisa Villanueva-Peñacarrillo
出处
期刊:Peptides
[Elsevier]
日期:1995-01-01
卷期号:16 (2): 225-229
被引量:62
标识
DOI:10.1016/0196-9781(94)00175-8
摘要
We have found [125I]glucagon-like peptide-1(7-36)-amide-specific binding activity in rat skeletal muscle plasma membranes, with an estimated M(r) of 63,000 by cross-linking and SDS-PAGE. The specific binding was time and membrane protein concentration dependent, and displaceable by unlabeled GLP-1(7-36)-amide with an ID50 of 3 x 10(-9) M of the peptide; GLP-1(1-36)-amide also competed, whereas glucagon and insulin did not. GLP-1(7-36)-amide did not modify the basal adenylate cyclase activity in skeletal muscle plasma membranes. These data, together with our previous finding of a potent glycogenic effect of GLP-1(7-36)-amide in rat soleus muscle, and also in isolated hepatocytes, which was not accompanied by a rise in the cell cyclic AMP content, lead use to believe that the insulin-like effects of this peptide on glucose metabolism in the muscle could be mediated by a type of receptor somehow different to that described for GLP-1 in pancreatic B cells, where GLP-1 action is mediated by the cyclic AMP-adenylate cyclase system.
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