志贺毒素
柠檬酸杆菌
微生物学
病菌
生物
志贺样毒素
毒素
柠檬酸杆菌
大肠杆菌
病毒学
毒力
细菌
肠杆菌科
基因
遗传学
生物化学
作者
Lynn Flowers,Songtao Hu,Anishma Shrestha,Amanda J. Martinot,John M. Leong,Marcia S. Osburne
出处
期刊:Springer eBooks
[Springer Nature]
日期:2021-01-01
卷期号:: 381-397
被引量:1
标识
DOI:10.1007/978-1-0716-1339-9_19
摘要
Shiga toxin-producing E. coli (STEC) is a common foodborne pathogen in developed countries. STEC generates “attaching and effacing” (AE) lesions on colonic epithelium, characterized by effacement of microvilli and the formation of actin “pedestals” beneath intimately attached bacteria. In addition, STEC are lysogenized with a phage that, upon induction, can produce potent Shiga toxins (Stx), potentially leading to both hemorrhagic colitis and hemolytic uremic syndrome. Investigation of the pathogenesis of this disease has been challenging because STEC does not readily colonize conventional mice.Citrobacter rodentium (CR) is a related mouse pathogen that also generates AE lesions. Whereas CR does not produce Stx, a murine model for STEC utilizes CR lysogenized with an E. coli-derived Stx phage, generating CR(Φstx), which both colonizes conventional mice and readily gives rise to systemic disease. We present here key methods for the use of CR(Φstx) infection as a highly predictable murine model for infection and disease by STEC. Importantly, we detail CR(Φstx) inoculation by feeding, determination of pathogen colonization, production of phage and toxin, and assessment of intestinal and renal pathology. These methods provide a framework for studying STEC-mediated systemic disease that may aid in the development of efficacious therapeutics.
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