Cloning and Sequence Analyzing of Sox 9 Genes' HMG Section of C. carpio

To investigate the features and functions of Sox 9 genes which are related to sex-determination and bony-tissue-development in C.carpio,several CcSox 9 genes were cloned from large quantity of individuals of several usual C.carpio varieties and wild Yellow River C.carpio.Sequences,including promoter-sequences,polymorphism,of these CcSox 9 genes and other CcSox 9 genes cloned by other domestic and foreign areas were analyzed.The results revealed that at least 5 active CcSox 9 genes and a pseudo gene PCcSox 9 were found.Abundant polymorphisms,including sequence polymorphisms and length polymorphisms,were found in these genes' coding sequences,especially introns.At least one promoter sequence can be found in each active CcSox 9 gene's intron.Pseudo gene PCcSox 9 has no intron and several terminal codons were found in its coding sequence.