Objective To analyze the TORCH screening results retrospectively of women at childbearing age in Guangzhou city, which can help us to know the epidemiological features of TORCH infection and provide basis for the prevention in the prenatal and postnatal stage. Methods Using indirect enzyme linked immunosorbent assay (ELISA), 3 640 women at childbearing age were performed prenatal screening test of ‘TORCH-IgM and IgG antibodies’ or ‘TORCH-IgM antibodies’ at the First Affilated Hospital of Jinan University from January 2009 to May 2011. Results The TORCH-IgM positive rate is 2.29% in ‘TORCH-IgM and IgG antibodies’ test, while the positive rate of TORCH-IgM in ‘TORCH-IgM antibody’ test is 1.59%. Totally, the TORCH-IgM positive rate is 2.03%. In ‘TORCH-IgM and IgG antibody’ test, the positive rate of Tox-IgG is 3.49%, RV-IgG is 69.6%, CMV-IgG is 83.3% and HSV-II-IgG is 71.0%. Conclusion Retrospective analysis showed that positive rates of TORCH-IgM and TORCH-IgG in women of childbearing age in Guangzhou city are relatively lower than the rates reported elsewhere previously. The reason may be due to the hygienic improvement in recent years.