Effects of aldosterone on calcium homeostasis and electrophysiological features on cardiocytes

Objective:To investigate the effect of aldosterone on calcium homeostasis and electrophysiological features on cardiocytes.Method:Single ventricular myocytes were isolated by enzymatic dissociation method.Isolated adult rat ventricular myocytes exposed for 48 hours to aldosterone 100 nmol/L,delayed after depolarizations(DAD) and triggered activity were recorded by using whole cell patch clamp technique.Calcium spark were recorded by using confocal microscopy.Result: An increase in the occurrence of DAD and triggered activity during action potential recordings were presented in aldosterone group.The frequency of DAD was 24/30 in aldosterone group and 7/30 in control group.The occurrence and redistribution to long-lasting and broader populations of spontaneous calcium sparks increased.Conclusion:Aldosterone may be disturb calcium homeostasis on cardiocytes which contributes to cardiac arrhythmia.