化学
检出限
碳纳米管
共轭体系
伏安法
再现性
纳米技术
抗体
循环伏安法
生物物理学
电极
电化学
胶体金
色谱法
纳米颗粒
有机化学
物理化学
材料科学
生物
免疫学
聚合物
作者
Rui Zhang,Shan Wang,Xiaoming Huang,Yihang Yang,Haitao Fan,Fan Yang,Jie Li,Xushuai Dong,Shaobin Feng,Periasamy Anbu,Subash C.B. Gopinath,Tao Xin
标识
DOI:10.1016/j.aca.2019.10.012
摘要
α-synuclein is a predominantly expressing neuronal protein for understanding the neurodegenerative disorders. A diagnosing system with aggregated α-synuclein encoded by SNCA gene is necessary to make the precautionary treatment against Parkinson's disease (PD). Herein, gold-nanourchin conjugated anti-α-synuclein antibody was desired as the probe and seeded on single-walled carbon nanotube (SWCN) integrated interdigitated electrode (IDE). The surface morphology of SWCN-modified IDE and gold urchin-antibody conjugates were observed under FESEM, FETEM and AFM, the existing elements were confirmed. Voltammetry analysis revealed that the limit of fibril-formed α-synuclein detection was improved by 1000 folds (1 fM) with gold-nanourchin-antibody modified surface, compared to the surface with only antibody (1 pM). Validating the interaction of α-synuclein by Enzyme-linked Immunosorbent Assay was displayed the detection limit as 10 pM. IDE has a good reproducibility and a higher selectivity on α-synuclein as evidenced by the interactive analysis with the control proteins, PARK1 and DJ-1.
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