Abstract Optimizing the fermentation conditions of Bacillus licheniformis and increasing the yield of Bacillus licheniformis to better serve agriculture is a very important research. The purpose of this experiment was to investigate the optimal fermentation conditions for increasing the production of Bacillus licheniformis. Through a series of investigations on Bacillus licheniformis, the system has learned the optimization method of the culture medium and the analysis method of the experimental data, and strengthened the basic experimental operation skills to lay the foundation for future experimental exploration.