医学
基质血管部分
富血小板血浆
骨愈合
脂肪组织
间质细胞
病理
成骨细胞
髓内棒
破骨细胞
骨髓
内科学
内分泌学
泌尿科
外科
血小板
受体
化学
体外
生物化学
作者
Atilla Adnan Eyuboglu,Enver Arpaci,Abbas Albayati,Ahmet Uysal,Aysen Terzi,Sema Bozalioglu,Hale Turnaoglu,Cenk Balcik,Burak Ozkan,Nilgün Markal Ertaş
出处
期刊:Annals of Plastic Surgery
[Ovid Technologies (Wolters Kluwer)]
日期:2020-09-01
卷期号:85 (3): 316-323
标识
DOI:10.1097/sap.0000000000002396
摘要
Background Chronic kidney disease (CKD) impairs osteoblast/osteoclast balance and damages bone structure with diminished mineralization and results in bone restoration disorders. In this study, we investigate the effects of adipose-derived stromal vascular fraction and platelet-rich plasma (PRP) on bone healing model in rats with CKD. Methods Sprague-Dawley rats were separated into 4 groups. All groups except group I (healthy control) had CKD surgery using 5/6 nephrectomy model. All groups had intramedullary pin fixation after receiving bone fracture using drilling tools. Group II rats were used as control group for CKD. Group III rats received PRP treatment on fracture site. Group IV rats received PRP and stromal vascular fraction treatment on fracture site.Weight loss and blood samples were followed at the time of kidney surgery, third, sixth, and 12th weeks. Bone healing and callus formations were compared, biomechanically, radiologically, histopathologically, and immunohistochemically. Osteoblastic transformation of stem cells was assessed with DiI staining. Results Negative effects of CKD on bone healing were reduced by increasing mechanical, histological, radiological, and biochemical properties of the bone with stromal vascular fraction and PRP treatments. Although thickness of callus tissue delayed bone healing process, it also enhanced biomechanical features and bone tissue organization. Conclusions Platelet-rich plasma and adipose-derived stromal vascular fraction treatments were effective for bone healing in animal model, which can be promising for clinical trials.
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