CD146号
流式细胞术
免疫球蛋白超家族
细胞
细胞粘附分子
免疫组织化学
体外
染色
分子生物学
细胞质
医学
细胞生物学
作者
Niansong Wang,Ying Fan,Peihong Ni,Rui Wang,Xu Gao,Xue Qin,Lingquan Tang
出处
期刊:PubMed
日期:2008-11-27
卷期号:21 (6): 931-40
被引量:5
摘要
Background CD146 is a cell adhesion molecule belonging to the immunoglobulin superfamily. It plays a critical role in cell proliferation and activation, and contributes to inflammatory reactions. We studied the association between CD146 and renal tubular epithelial cells (RTECs) under normal (NG) and high glucose (HG) to understand its role in the pathogenesis of diabetic nephropathy. Methods Human proximal tubular epithelial (HK-2) cells were cultured in different glucose media (NG5mM, HG15mM and HG40mM). CD146 mRNA expression was determined by RT-PCR. CD146 protein expression was assessed by fluorescence immunoassay, enzymatic immunohistochemistry and flow cytometry. A soluble form of CD146 was evaluated by ELISA. Results We found weak expression of CD146 mRNA and protein in the NG group. Faint labeling of CD146 protein was detected mainly at the cell-cell junction, with sparse staining in cytoplasm and nucleus. In the HG group, both membrane and intracellular expression of CD146 were significantly increased by high glucose, while osmotic pressure had no effect on CD146 expression in cultured HK-2 cells. A soluble form of CD146, sCD146, was detected in the supernatant of HK-2 cells in the NG group which was elevated in the HG40mM group (29.33 -/+ 1.22 ng/mL vs. 61.87 -/+ 9.10 ng/mL, p Conclusion CD146 might be a novel marker for RTECs, correlating with cell proliferation and cell-cell conjunctions. High glucose up-regulates both CD146 and sCD146 expression. CD146 may be a biomarker of activated RTECs, and sCD146 may have clinical value for determining severity and prognosis of diabetic nephropathy.
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