寡核苷酸
化学
DNA
感应(电子)
纳米结构
基因
基因表达
生物标志物
表型
遗传增强
分子生物学
计算生物学
细胞生物学
纳米技术
癌症研究
生物
生物化学
材料科学
物理化学
作者
Lok Ting Chu,Hoi Kwan Kwong,Chenyu Cui,Ting‐Hsuan Chen
出处
期刊:Talanta
[Elsevier]
日期:2024-03-01
卷期号:269: 125399-125399
标识
DOI:10.1016/j.talanta.2023.125399
摘要
Antisense oligonucleotide (ASO) is a powerful agent for gene therapy, designed to form complementary pairs with specific mRNA to inhibit gene expression. However, low specificity limits its potential. To overcome this challenge, we developed a Y-shape DNA nanostructure that enhances the specificity in ASO-based treatment by introducing a detection trigger. The design incorporates the phenotype-specific miR21 activation and the sequential release of Bcl2 ASO. As a result, our Y-shape DNA nanostructure downregulates >50 % Bcl2 mRNA expression and induces >60 % cell death in breast cancer cells. Meanwhile, this approach shows no obvious damage to the non-cancerous cells, indicating the therapeutic potential as a theranostics agent in precision medicine with the combination of biomarker sensing and treatment. Overall, our Y-shape DNA nanostructure serves as a promising strategy providing potential in customized conformation design with specific target sequences in gene therapy.
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