拟南芥
精胺
谷胱甘肽还原酶
亚精胺
拟南芥
超氧化物歧化酶
生物
谷胱甘肽
植物
化学
生物化学
抗氧化剂
酶
谷胱甘肽过氧化物酶
突变体
基因
作者
Bing Gao,Xiangyue Liu,Zhenghong Feng,Jianhui Wu,Jinghong Wang
出处
期刊:Chemosphere
[Elsevier]
日期:2023-11-01
卷期号:342: 140194-140194
标识
DOI:10.1016/j.chemosphere.2023.140194
摘要
Potentilla sericea is resistant and tolerates rough management. It is an excellent garden groundcover for ecological restoration and soil consolidation for slope protection. Polyamines have functions such as promoting tissue growth and physiological resistance, while spermine synthase catalyzes the production of spermine. The PsSPMS gene from Potentilla sericea was cloned and transformed into Arabidopsis thaliana to study the response of transgenic Arabidopsis thaliana to cadmium stress. The results showed that the contents of spermidine, spermine as well as glutathione were higher in PsSPMS overexpressing Arabidopsis thaliana than the control, while the contents of putrescine were less than the control. Net photosynthetic rate, stomatal conductance, chlorophyll content, water use efficiency, electron transfer rate, PSII-related parameters, proline content, superoxide dismutase, and glutathione reductase activities were higher in PsSPMS overexpressing Arabidopsis thaliana than the control, while malondialdehyde, superoxide anion, and hydrogen peroxide contents were lower than the control. Correlation analysis showed significant differences between the indicators (P < 0.05 and P < 0.01). Expression of AtSPMS, AtSPD3, AtGSH2 and AtGR in transgenic Arabidopsis thaliana was higher than that of the control. Therefore, this study provides a genetic reference for the cultivation of cadmium-tolerant plants through genetic engineering and lays the foundation for further research on cadmium-tolerant Potentilla sericea.
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