血小板生成素
骨髓
巨核细胞
造血
血小板
活体显微镜检查
病理
巨核细胞生成
脾脏
生物
流式细胞术
髓腔
祖细胞
干细胞
免疫学
细胞生物学
体内
医学
生物技术
作者
Nathan Asquith,Estelle Carminita,Virginia Camacho,Antonio Rodriguez-Romera,David Stegner,Daniela Freire,Isabelle C. Becker,Kellie R. Machlus,Abdullah O. Khan,Joseph E. Italiano
出处
期刊:Blood
[American Society of Hematology]
日期:2023-10-25
被引量:1
标识
DOI:10.1182/blood.2023020895
摘要
Megakaryocytes generate thousands of platelets over their lifespan. More recently, roles in infection and inflammation have been reported. These findings have driven a study of extra-medullary thrombopoiesis, and megakaryocytes have been increasingly reported within the spleen and lung. However, the relative abundance of megakaryocytes in these organs compared to the bone marrow and the scale of their contribution to the platelet pool in steady state remains controversial. We investigated the relative abundance of megakaryocytes in the adult murine bone marrow, spleen, and lung using whole-mount light sheet and quantitative histological imaging, flow cytometry, intravital imaging, and an assessment of scRNA-seq repositories. Flow cytometry revealed significantly higher numbers of hematopoietic stem and progenitor cells and megakaryocytes in the murine bone marrow compared to spleens or perfused lungs. Two-photon intravital and light-sheet microscopy, as well as cryosections confirmed these findings. Moreover, ex-vivo cultured megakaryocytes from the bone marrow subject to static or microfluidic platelet production assays had a higher capacity for proplatelet-formation than MKs from other organs. Analysis of previously published murine and human scRNA-seq datasets revealed that only a marginal fraction of megakaryocyte-like cells can be found within the lung and most likely only marginally contribute to platelet production in the steady state.
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