生化工程
合成生物学
稳健性(进化)
生物技术
小分子
计算生物学
生物过程
计算机科学
生物
生物化学
工程类
基因
古生物学
作者
Bharat Singh,Ankit Kumar,Adesh K. Saini,Reena V. Saini,Rahul Thakur,Shakeel Ahmed Mohammed,Hardeep Singh Tuli,Vijai Kumar Gupta,Mohammed Y. Areeshi,Hani Faidah,Naif A. Jalal,Shafiul Haque
标识
DOI:10.1080/02648725.2023.2177039
摘要
High demand of bioactive molecules (food additives, antibiotics, plant growth enhancers, cosmetics, pigments and other commercial products) is the prime need for the betterment of human life where the applicability of the synthetic chemical product is on the saturation due to associated toxicity and ornamentations. It has been noticed that the discovery and productivity of such molecules in natural scenarios are limited due to low cellular yields as well as less optimized conventional methods. In this respect, microbial cell factories timely fulfilling the requirement of synthesizing bioactive molecules by improving production yield and screening more promising structural homologues of the native molecule. Where the robustness of the microbial host can be potentially achieved by taking advantage of cell engineering approaches such as tuning functional and adjustable factors, metabolic balancing, adapting cellular transcription machinery, applying high throughput OMICs tools, stability of genotype/phenotype, organelle optimizations, genome editing (CRISPER/Cas mediated system) and also by developing accurate model systems via machine-learning tools. In this article, we provide an overview from traditional to recent trends and the application of newly developed technologies, for strengthening the systemic approaches and providing future directions for enhancing the robustness of microbial cell factories to speed up the production of biomolecules for commercial purposes.
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