Probiotic Fermentation of Kelp Enzymatic Hydrolysate Promoted its Anti-Aging Activity in D-Galactose-Induced Aging Mice by Modulating Gut Microbiota

In order to investigate the anti-aging effect of probiotic-fermented kelp, a nutrient-rich marine brown algae, probiotics mixture of Lactobacillus reuteri, Pediococcus pentosaceus and Lactobacillus acidophilus were used for the fermentation in this study. The effects of probiotic-fermented kelp enzymatic hydrolysate culture (KMF), probiotic-fermented kelp enzymatic hydrolysate supernatant (KMFS) and probiotic-fermented kelp enzymatic hydrolysate bacteria suspension (KMFP) in aging mice model with D-galactose-induced were comprehensively compared. The results showed that KMF, KMFS, and KMFP could prevent D-galactose-induced elevation of malondialdehyde (MDA) levels in serum and brain tissue of aging mice, and increase the levels of superoxide dismutase (SOD), total antioxidant capacity (T-AOC) and catalase (CAT). At the same time, they could improve the cell structure of mouse brain, liver and intestinal tissue. In addition, KMF, KMFS and KMFP intervention could regulate mRNA and protein expression levels of genes responsible for aging. Furthermore, the abundance and composition of the gut microbiota can be modulated in KMF, KMFS and KMFP groups. These results suggest that KMF, KMFS, and KMFP have the potential to modulate gut microbiota imbalance and aging-related genes for anti-aging.