纳米技术
生物分子
酵母
合成生物学
拉曼散射
材料科学
生物传感器
化学
计算生物学
生物物理学
生物
拉曼光谱
生物化学
光学
物理
作者
Junrong Li,Christopher B. Howard,Shuvashis Dey,Kym Lowry,David M. Whiley,Simon Puttick,Stephen Rose,Richard J. Lobb,Alain Wuethrich,Selvakumar Edwardraja,Matt Trau
标识
DOI:10.1038/s41565-023-01415-1
摘要
Accurate and early detection of biomarkers provides the molecular evidence for disease management, allowing prompt actions and timely treatments to save lives. Multivalent biomolecular interactions between the probe and biomarker as well as controlled probe orientation on material surfaces are keys for highly sensitive detection. Here we report the bioengineering of programmable and multifunctional nanoprobes, which can provide rapid, specific and highly sensitive detection of emerging diseases in a range of widely used diagnostic systems. These nanoprobes composed of nanosized cell wall fragments, termed as synthetic bionanofragments (SynBioNFs), are generated by the fragmentation of genetically programmed yeast cells. SynBioNFs display multiple copies of biomolecules for high-affinity target binding and molecular handles for the precisely orientated attachment on surfaces used in diagnostic platforms. SynBioNFs are demonstrated for the capture and detection of SARS-CoV-2 virions using multiple diagnostic platforms, including surface-enhanced Raman scattering, fluorescence, electrochemical and colorimetric-based lateral flow systems with sensitivity comparable with the gold-standard reverse-transcription quantitative polymerase chain reaction.
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