Discovery and determination of misuse and chemotypes of Pogostemon cablin by liquid chromatography‐quadrupole time‐of‐flight mass spectrometry and liquid chromatography with diode‐array detector

Traditional Chinese medicine (TCM) has garnered significant scientific interest in healthcare but faces increased regulatory scrutiny due to concerns about uncontrolled usage. This study focuses on characterizing Pogostemon cablin (PC) to mitigate potential misuse and identify chemotype differences. Leveraging untargeted metabolomics, we identified 222 distinctive features effectively differentiating PC from Agastache rugosa (AR), reducing misidentification risks. Pogostone and tilianin emerged as potential markers, leading to a high‐performance liquid chromatography‐diode array detection (HPLC‐DAD) method development for PC and AR discrimination. Evaluation of PC chromatograms revealed notable profile and pogostone level differences among samples, suggesting chemotype associations. Untargeted metabolic profiling identified 78 features with significant differences, highlighting 7,3′,4′‐tri‐O‐methyleriodictyol as a potential discriminatory marker between PC chemotypes. The developed HPLC‐DAD method quantified pogostone and 7,3′,4′‐tri‐O‐methyleriodictyol, effectively discriminating PC chemotypes. This platform differentiates PC and AR and distinguishes chemical types within PC, like pogostone‐type and patchoulol‐type. Applied to local TCM stores, it ensures PC authenticity. This approach addresses TCM control concerns, enhancing understanding and application of herbal medicine by providing insights into PC chemical composition and discrimination.