骨整合
材料科学
再生(生物学)
脚手架
钙
多孔性
结晶度
结晶
纳米技术
生物医学工程
化学工程
细胞生物学
复合材料
植入
生物
医学
冶金
外科
工程类
作者
Peng Wei,Jingyu Zhou,Shilang Xiong,Yi Feng,Kejun Xu,Min Lee,Hanrui Xi,Zhigang Zhou,Zhiqiang Qiu,Hantian Liu,Jing Zeng,Yayun Liu,Peng Qiu,Jian‐Guo Zhou,Shiwei Liu,Zhisheng Long,Jingtang Li,Long Xiong
标识
DOI:10.1021/acsami.3c17087
摘要
This study aims to overcome the drawbacks associated with hydroxyapatite (HAP) dense structures after sintering, which often result in undesirable features such as large grain size, reduced porosity, high crystallinity, and low specific surface area. These characteristics hinder osseointegration and limit the clinical applicability of the material. To address these issues, a new method involving the preparation of hollow hydroxyapatite (hHAP) microspheres has been proposed. These microspheres exhibit distinctive traits including weak crystallization, high specific surface area, and increased porosity. The weak crystallization aligns more closely with early mineralization products found in the human body and animals. Moreover, the microspheres' high specific surface area and porosity offer advantages for protein loading and facilitating osteoblast attachment. This innovative approach not only mitigates the limitations of conventional HAP structures but also holds the potential for improving the effectiveness of hydroxyapatite in biomedical applications, particularly in enhancing osseointegration. Three-dimensional printed hHAP/chitosan (CS) scaffolds with different hHAP concentration gradients were manufactured, and the physical and biological properties of each group were systematically evaluated. In vitro and in vivo experiments show that the hHAP/CS scaffold has excellent performance in bone remodeling. Furthermore, in-scaffold components, hHAP and CS were cocultured with bone marrow mesenchymal stem cells to explore the regulatory role of hHAP and CS in the process of bone healing and to reveal the cell-level specific regulatory network activated by hHAP. Enrichment analysis showed that hHAP can promote bone regeneration and reconstruction by recruiting calcium ions and regulating inflammatory reactions.
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