Characterization and 3D printing of a biodegradable polylactic acid/thermoplastic polyurethane blend with laccase-modified lignin as a nucleating agent

Polylactic acid (PLA) has been used in fused deposition method (FDM) based 3D printing for many years. Alkali lignin is an undervalued industrial by-product that could upgrade PLA's poor mechanical properties. This work presents a biotechnological approach consisting of a partial degradation of alkali lignin using Bacillus ligniniphilus laccase (Lacc) L1 for its use as a nucleating agent in a polylactic acid/thermoplastic polyurethane (PLA/TPU) blend. Results showed that adding enzymatically modified lignin (EL) increased the elasticity modulus to a maximum of 2.5-fold than the control and conferred a maximum biodegradability rate of 15 % after 6 months under the soil burial method. Furthermore, the printing quality rendered satisfactory smooth surfaces, geometries and a tunable addition of a woody color. These findings open a new door for using laccase as a tool to upgrade lignin's properties and its use as a scaffold in manufacturing more environmentally sustainable filaments with improved mechanical properties for 3D printing.