Direct Spectrophotometric Determination of Serum and Urinary Oxalate with Oxalate Oxidase

A new enzymatic method for direct photometric determination of oxalate in serum and urine is described, using oxalate oxidase.The resulting H 2 0 2 is measured with a coupled enzyme system of catalase and aldehyde dehydrogenase.Percentage recovery of added oxalate was 99 ± 4 in serum, and 98 ± 4 in urine (n = 10).Oxalate serum levels varied from 16.9 to 44.8 μηηοΙ/L Oxalate values can be determined within 20 minutes, without time consuming pretreatment of samples.The detection limit is 5 μπιοΐ/ΐ. Direkte spektrophotometrische Bestimmung von Serum-und Urin-Oxalat mit Oxalat-Oxidase