Expanding the catalytic repertoire of C–C bond–forming pyridoxal 5′-phosphate-dependent enzymes for noncanonical amino acid formation

Noncanonical amino acids (ncAAs) are pharmaceutically important molecules with diverse biological and chemical applications. Among various production strategies, biocatalytic synthesis using pyridoxal 5'-phosphate (PLP)-dependent enzymes has attracted considerable attention owing to their high stereoselectivity and versatility in forming new C-C bonds. Recent advances in enzyme engineering have further expanded the catalytic potential of PLP-dependent enzymes, enabling the tailored synthesis of target ncAAs. In this short review, we summarize recent developments in biocatalytic ncAA synthesis achieved through engineered PLP-dependent enzymes.