EFFECT OF β-AESCINATE ON NO METABOLISM IN BRAIN ISCHEMIA AND ISCHEMIA-REPERFUSION MODEL OF RATS

Objective To study the effect of β aescinate on NO metabolism in acute ischemia and ischemia reperfusion model of rat brain. Methods The acute ischemia and ischemia reperfusion model of rat brain was induced by blocking of four vessels. The rats were divided into seven groups: control group; 30 minute global ischemia group; 30 minute global ischemia+drug group; 30 minute global ischemia and 30 minute reperfusion group; 30 minute global ischemia and 30 minute reperfusion +drug group; 30 minute global ischemia and 5 day reperfusion group; 30 minute global ischemia and 5 day reperfusion group. β aescinate (10 mg/kg, iv) was injected before operation. The changes of the contents of NO, MDA and SOD in the cortex and serum were observed. Results Compared with the rats in the drug group, those in the ischemia group and ischemia reperfusion group showed a significant increase in concentrations of NO and MDA in the cortex and serum ( F=18.51-123.66, q=5.41- 29.59 , P 0.01), but a significant decrease in the level of SOD ( F=5.87, q=4.81-26.02, P 0.05). Conclusion β aescinate may influence NO metabolism and protect the neuron cells by means of anti free radicals.