The denitrification treatment of sewage produces not only the final product N2,but also the intermediate product of Nitrous Oxide(N2O),which is one of the important potent greenhouse gases.It has important theoretical and practical significance to understanding the production mechanism and influence factors of N2O.Influence factors of N2O accumulation during denitrification are summarized and analyzed in this paper,including C/N ratio,DO,pH value,temperature and NO2--N.The relationship between the nitrous oxide reductase(Nos) activity and N2O accumulation during denitrification has also been discussed.