In February 2010,three different sources of Macrobrachium rosenbergii larvae from private breeding farm of Jiangsu Province,Guangxi autonomous region and state level seed breeding farm of Macrobrachium rosenbergii in Nanning,were connected and used for juvenile culture.Dry larval volume method was adopted to calculate the juvenile amounts and larvae survival rate.Experiment results indicated that the survival rate of 3 different sources of Macrobrachium rosenbergii larval were 73.6%,65.2% and 90.4%,respectively,having distinct difference among them.And the survival rate of larval from state-level seed breeding farm of Macrobrachium rosenbergii in Nanning was the highest.Macrobrachium rosenbergii seedling must focus on quality of the larvae.