INDUCTION OF TETRAPLOID IN TURBOT SCOPHTHALMUS MAXIMUS

Turbot Scophthalmus maximus is an important cultured marine fish in North China.The induction of tetraploid turbot is important in germplasm enhancement for large-scale applications of triploid.However,there is almost no report on artificial induction of tetraploid turbot.In this study,the tetraploid turbot was induced by hydrostatic pressure shock to prevent embryo cleavage.The results show that the optimal initiation time for pressure shock was 15 min before the first cleavage at 14.8—15.5?C.The optimal treatment pressure and treatment duration were 67.5MPa and 6 min.The hatching rate and tetraploid rate could reach 72% and 70%,respectively.This paper provides basic data for production of fertile tetraploid adults in turbot.