BACILLUS THURINGIENSIS BIOASSAY METHODOLOGY

This review dealt with the importance and Popular procedures of the standardized bioassay for Bacillus thuringiensis. The standardized bioassay procedures for larvae of Lepidoptera includes: the diet - incorporation bioassay, the surface contamination assay, the hole - in - wax assay and the droplet assay. The standardized bioassay procedures for larvae of Diptera are the World Health Organization method and the U. S. method. However the bioassay for larvae of Coleoptera has not ho standardized. Though immunochemical assays have some defects, they are more rapid and simple than conventional bioassays.