Expression,Purification and Refolding of Recombinant Matrix Metalloproteinase MT5-MMP

The authors completed the induction expression of recombinant MT5-MMP in E.coli BL21(DE3) and harvested the protein as inclusion bodies.The molecular weight of the protein is about 21 000.In addition,we purified and refolded the recombinant protein using the chromatography method to obtain the catalytically active MT5-MMP.