Salt-soluble protein was obtained from defatted proso millet powder by NaCl solution extracted. We studied the effects of concentration of NaCl solution, extraction time and powder-to-salt solution ratio. After that we adopted L_9(3~3)orthogonal experiments to determine the optimal extraction condition, and found that the optimal extraction condition were extraction time 30min, powder-to-salt solution ratio.1∶9 and concentration of NaCl solution 2%.Under this condition,yield of protein was 3.96%.