免疫系统
免疫学
肺炎
生物
CD8型
外周血单个核细胞
病毒学
转录组
人口
疾病
医学
基因
遗传学
基因表达
病理
内科学
环境卫生
体外
作者
Alex R. Schuurman,Tom D. Y. Reijnders,Anno Saris,Ivan Ramirez‐Moral,Michiel Schinkel,Justin de Brabander,Christine van Linge,Louis Vermeulen,Brendon P. Scicluna,W. Joost Wiersinga,Felipe A. Vieira Braga,Tom van der Poll
出处
期刊:eLife
[eLife Sciences Publications, Ltd.]
日期:2021-08-23
卷期号:10
被引量:12
摘要
The exact immunopathophysiology of community-acquired pneumonia (CAP) caused by SARS-CoV-2 (COVID-19) remains clouded by a general lack of relevant disease controls. The scarcity of single-cell investigations in the broader population of patients with CAP renders it difficult to distinguish immune features unique to COVID-19 from the common characteristics of a dysregulated host response to pneumonia. We performed integrated single-cell transcriptomic and proteomic analyses in peripheral blood mononuclear cells from a matched cohort of eight patients with COVID-19, eight patients with CAP caused by Influenza A or other pathogens, and four non-infectious control subjects. Using this balanced, multi-omics approach, we describe shared and diverging transcriptional and phenotypic patterns—including increased levels of type I interferon-stimulated natural killer cells in COVID-19, cytotoxic CD8 T EMRA cells in both COVID-19 and influenza, and distinctive monocyte compositions between all groups—and thereby expand our understanding of the peripheral immune response in different etiologies of pneumonia.
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